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Home >> Antibodies >> Transducin-like enhancer protein 1 Antibody / TLE1

Transducin-like enhancer protein 1 Antibody / TLE1 [clone TLE1/8007R] (V5294)

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  Catalog No Formulation Size Price (USD)  
Image V5294-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V5294-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V5294SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human prostate carcinoma tissue with Transducin-like enhancer protein 1 antibody (clone TLE1/8007R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human prostate carcinoma tissue with Transducin-like enhancer protein 1 antibody (clone TLE1/8007R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human bladder tissue with Transducin-like enhancer protein 1 antibody (clone TLE1/8007R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name TLE1/8007R
Purity Protein A/G affinity
UniProt Q04724
Localization Nucleus
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This TLE1 antibody is available for research use only.
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Description

Key players in the Notch pathway are the TLE genes, which are human homologs of the Drosophila groucho gene. Groucho is a transcriptional repressor that plays a key role in neurogenesis, segmentation and sex determination. Transducin-like enhancer protein1 (TLE1) is a protein that is encoded by the TLE1 gene and is involved in control of hematopoiesis, neuronal, and terminal epithelial differentiation. Positive immunohistochemical nuclear staining with anti-TLE-1 has been shown to be a useful addition to an IHC panel when differentiating synovial sarcoma from other soft tissue malignancies.

Application Notes

Optimal dilution of the Transducin-like enhancer protein 1 antibody should be determined by the researcher.

Immunogen

A recombinant human protein fragment (aa 175-338) was used as the immunogen for the Transducin-like enhancer protein 1 antibody.

Storage

Aliquot the Transducin-like enhancer protein 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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