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Home >> Antibodies >> TRAIP Antibody / TRAF-interacting protein / TRIP

TRAIP Antibody / TRAF-interacting protein / TRIP (RQ7536)

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  Catalog No Formulation Size Price (USD)  
Image RQ7536 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human HeLa cells with TRAIP antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human HeLa cells with TRAIP antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human Daudi, 2) human U-251, 3) rat brain and 4) mouse brain tissue lysate with TRAIP antibody. Predicted molecular weight ~53 kDa.
Flow cytometry testing of human SiHa cells with TRAIP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TRAIP antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9BWF2
Localization Nuclear, cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This TRAIP antibody is available for research use only.
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  • Applications : IHC, ELISA (peptide)
    Reactivity : Human

Description

TRAF-interacting protein is a protein that in humans is encoded by the TRAIP gene. This gene encodes a protein that contains an N-terminal RING finger motif and a putative coiled-coil domain. A similar murine protein interacts with TNFR-associated factor 1 (TRAF1), TNFR-associated factor 2 (TRAF2), and cylindromatosis. The interaction with TRAF2 inhibits TRAF2-mediated nuclear factor kappa-B, subunit 1 activation that is required for cell activation and protection against apoptosis.

Application Notes

Optimal dilution of the TRAIP antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids M1-S469) was used as the immunogen for the TRAIP antibody.

Storage

After reconstitution, the TRAIP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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