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TNF Receptor-Associated Factor 4, also called CART1 or MLN62, is a protein that in humans is encoded by the TRAF4 gene. It is a member of the TNF receptor associated factor (TRAF) family, a family of scaffold proteins. Tomasetto et al. (1995) identified genes that are overexpressed in breast cancer by differential screening of cDNAs from breast cancer-derived metastatic axillary lymph nodes. The TRAF4 cDNA, which they designated MLN62, was mapped to 17q11-q12 by radioisotopic in situ hybridization. To investigate the biologic function of TRAF4, Regnier et al. (2002) generated Traf4-deficient mice by gene disruption.
The stated application concentrations are suggested starting amounts. Titration of the TRAF4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
An amino acid sequence from the C-terminus of human TNF Receptor-Associated Factor 4 (PKFISHQDIRKRNYVRDD) was used as the immunogen for this TRAF4 antibody (100% homologous in human, mouse and rat).
After reconstitution, the TRAF4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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