TNF Receptor Antibody / TNFR1 / TNFRSF1A (RQ4614)

IHC staining of FFPE mouse lung with TNF Receptor antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

IHC staining of FFPE mouse liver with TNF Receptor antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

IHC staining of FFPE mouse kidney with TNF Receptor antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Flow cytometry testing of human U937 cells with TNF Receptor antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TNF Receptor antibody.

Western blot testing of 1) human HeLa, 2) human K562, 3) human Caco-2, 4) rat liver, 5) mouse small intestine, 6) mouse liver and 7) mouse HEPA1-6 lysate with TNF Receptor antibody at 0.5ug/ml. Predicted molecular weight ~51 kDa.