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Home >> Antibodies >> Recombinant TNF-alpha Antibody / Rabbit Monoclonal

Recombinant TNF-alpha Antibody / Rabbit Monoclonal [clone RMTNFA-1] (V7220)

  Catalog No Formulation Size Price (USD)  
V7220-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 379

V7220-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199

V7220SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 399

V7220IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 379
IHC testing of human pancreas with recombinant TNF alpha antibody (clone RMTNFA-1). Required HIER: steam sections in 10mM Citrate buffer, pH 6.0, for 10-20 min.
IHC: Formalin-fixed, paraffin-embedded human histiocytoma stained with TNF alpha antibody (clone RMTNFA-1). Required HIER: steam sections in 10mM Citrate buffer, pH 6.0, for 10-20 min.
Availability 1-3 business days
Species Reactivity Human. Other species not known.
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, k
Clone Name RMTNFA-1
Purity Protein A affinity chromatography
UniProt P01375
Localization Cytoplasmic and extracellular (secreted)
Applications Immunofluorescence : 5-10ug/ml
Immunohistochemistry (FFPE) : 5-10ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This recombinant TNF-alpha antibody is available for research use only.

Description

Tumor necrosis factor alpha is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons.

The primary role of TNF is in the regulation of immune cells. TNFa, being an endogenous pyrogen, is able to induce fever, apoptotic cell death, cachexia, inflammation and to inhibit tumorigenesis and viral replication and respond to sepsis via IL1 & IL6 producing cells. Dysregulation of TNF production has been implicated in a variety of human diseases including Alzheimer's disease, cancer, major depression, Psoriasis and inflammatory bowel disease (IBD). [Wiki]

Application Notes

Optimal dilution of the recombinant TNF-alpha antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human protein was used as the immunogen for the recombinant TNF-alpha antibody.

Storage

Store the recombinant TNF-alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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