TMC7 Antibody / Transmembrane channel-like protein 7 (RQ7558)

Immunofluorescent staining of FFPE human intestinal cancer with TMC7 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

IHC staining of FFPE human colorectal adenocarcinoma tissue with TMC7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human liver cancer tissue with TMC7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of 1) human HaCaT, 2) human HepG2, 3) human U-87 MG, 4) rat testis, 5) rat C6 and 6) mouse RAW264.7 cell lysate with TMC7 antibody. Predicted molecular weight ~84 kDa but may be observed at higher molecular weights due to glycosylation.

Flow cytometry testing of human SiHa cells with TMC7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TMC7 antibody.