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Home >> Antibodies >> TGF alpha Antibody

TGF alpha Antibody [clone 1E8-G6] (V2267)

  Catalog No Formulation Size Price (USD)  
V2267-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 329

V2267-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 129

V2267SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 359
Formalin-fixed, paraffin-embedded human testicular carcinoma stained with TGF-alpha antibody (1E8-G6)
Species Reactivity Human, Rabbit and Zebrafish. Other species not known.
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgM, kappa
Clone Name 1E8-G6
Purity PEG precipitation
Buffer 1X PBS, pH 7.4
Gene ID 7039
Localization Cytoplasmic and secreted
Applications Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2ug/ml
IHC (Formalin-paraffin) : 1-2ug/ml for 30 min at RT (1)
Limitations This TGF alpha antibody is available for research use only.

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Description

TGF alpha is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. Staining with the antibody is completely blocked by the peptide used for raising this antibody.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunogen

A 10-amino acid synthetic peptide (aa 34-43) from human TGF alpha was used as the immunogen for this antibody.

Storage

Store the TGF alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

EGF-like TGF; ETGF; TFGA; TGF Type 1; TGFA; Wa1; Waved 1, TGF alpha antibody

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