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Home >> Antibodies >> TEK Antibody / TIE2

TEK Antibody / TIE2 (RQ4918)

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  Catalog No Formulation Size Price (USD)  
Image RQ4918 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) placenta, 2) Caco-2, 3) human HeLa, 4) rat liver and 5) mouse liver lysate with TEK antibody at 0.5ug/ml. Predicted molecular weight: ~126 kDa but may be observable at 130-165 kDa.
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Western blot testing of 1) placenta, 2) Caco-2, 3) human HeLa, 4) rat liver and 5) mouse liver lysate with TEK antibody at 0.5ug/ml. Predicted molecular weight: ~126 kDa but may be observable at 130-165 kDa.
IHC staining of FFPE human breast cancer with TEK antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human placenta with TEK antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with TEK antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE mouse lung with TEK antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE mouse heart with TEK antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE rat heart with TEK antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q02763
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This TEK antibody is available for research use only.
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Description

TIE2, also known as TEK tyrosine kinase, is mapped to 9p21.2. This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Immunoblotting showed that TIE2 expression was increased by thyroid-stimulating hormone and agents that increased intracellular cAMP. HSCs expressing the receptor tyrosine kinase TIE2 are quiescent and antiapoptotic and comprise a side population of HSCs that adhere to osteoblasts in the bone marrow niche.

Application Notes

Optimal dilution of the TEK antibody should be determined by the researcher.

Immunogen

Amino acids EALMNQHQDPLEVTQDVTREWAKKVVWK from the human protein were used as the immunogen for the TEK antibody.

Storage

After reconstitution, the TEK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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