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Home >> Antibodies >> TDG Antibody / Thymine-DNA glycosylase

TDG Antibody / Thymine-DNA glycosylase (RQ7493)

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  Catalog No Formulation Size Price (USD)  
Image RQ7493 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human PC-3 cells with TDG antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TDG antibody.
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Flow cytometry testing of human PC-3 cells with TDG antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TDG antibody.
Flow cytometry testing of human HL60 cells with TDG antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TDG antibody.
Western blot testing of 1) human HeLa, 2) monkey COS-7 and 3) human placental tissue lysate with TDG antibody. Predicted molecular weight ~46 kDa.
Availability 1-3 business days
Species Reactivity Human, Monkey
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q13569
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This TDG antibody is available for research use only.
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Description

G/T mismatch-specific thymine DNA glycosylase is an enzyme that in humans is encoded by the TDG gene. The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12.

Application Notes

Optimal dilution of the TDG antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids Q15-R370) was used as the immunogen for the TDG antibody.

Storage

After reconstitution, the TDG antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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