TCP1 epsilon Antibody / CCT5 (R32402)

Western blot testing of 1) rat testis and 2) human HeLa lysate with TCP1 epsilon antibody. Expected molecular weight ~60 kDa.

IHC testing of FFPE human intestinal cancer tissue with TCP1 epsilon antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.

IHC testing of FFPE mouse ovary with TCP1 epsilon antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.

IHC testing of FFPE rat ovary with TCP1 epsilon antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.

ICC testing of FFPE human LOVO cells with TCP1 epsilon antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.

Flow cytometry testing of human A431 cells with TCP1 epsilon antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TCP1 epsilon antibody.

Immunofluorescent staining of FFPE human U-2 OS cells with TCP1 epsilon antibody (green) at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of 1) human HeLa, 2) human MCF7, 3) human SiHa, 4) human A549, 5) human 293T, 6) human Caco-2, 7) human PC-3, 8) human U-2 OS, 9) rat testis, 10) mouse testis and 11) mouse liver tissue lysate with TCP1 epsilon antibody. Expected molecular weight ~60 kDa.