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Home >> Antibodies >> TBP Antibody

TBP Antibody (F49784)

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  Catalog No Formulation Size Price (USD)  
Image F49784-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F49784-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Fluorescent confocal image of U251 cell stained with TBP antibody at 1:25. TBP immunoreactivity is localized to the nucleus.
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Fluorescent confocal image of U251 cell stained with TBP antibody at 1:25. TBP immunoreactivity is localized to the nucleus.
TBP antibody western blot analysis in (1) K562 and (2) U251 lysate. Observed molecular weight: 35-43 kDa.
IHC analysis of FFPE human testis tissue stained with TBP antibody
TBP antibody flow cytometry analysis of HL-60 cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
  • Fluorescent confocal image of U251 cell stained with TBP antibody at 1:25. TBP immunoreactivity is localized to the nucleus.
  • TBP antibody western blot analysis in (1) K562 and (2) U251 lysate. Observed molecular weight: 35-43 kDa.
  • IHC analysis of FFPE human testis tissue stained with TBP antibody
  • TBP antibody flow cytometry analysis of HL-60 cells (green) compared to a <a href=negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis." title="TBP antibody flow cytometry analysis of HL-60 cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis." onClick="showhideiamges('image4','imagelargedata4',4,4)">
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Bovine, Chicken, Mouse, Primate, Xenopus, Zebrafish
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P20226
Applications Western blot : 1:1000
Immunofluorescence : 1:10-1:50
IHC (Paraffin) : 1:50-1:100
Flow Cytometry : 1:10-1:50
Limitations This TBP antibody is available for research use only.
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Description

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. A distinctive feature of TBP is a long string of glutamines in the N-terminal. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. Mutations that expand the number of CAG repeats encoding this polyglutamine tract, and thus increase the length of the polyglutamine string, are associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease.

Application Notes

Titration of the TBP antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 210-239 from the human protein was used as the immunogen for this TBP antibody.

Storage

Aliquot the TBP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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