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Home >> Antibodies >> TBP Antibody / TATA-box-binding protein

TBP Antibody / TATA-box-binding protein [clone 830CT4.3.3] (F52225)

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  Catalog No Formulation Size Price (USD)  
Image F52225-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F52225-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of 1) human 293, 2) (h) HeLa, 3) (h) MDA-MB-231, 4) rat H-4-II-E, 5) (h) HCT-116 and 6) mouse C2C12 cell lysate with TBP antibody at 1:2000. Expected molecular weight: 35-43 kDa.
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Western blot testing of 1) human 293, 2) (h) HeLa, 3) (h) MDA-MB-231, 4) rat H-4-II-E, 5) (h) HCT-116 and 6) mouse C2C12 cell lysate with TBP antibody at 1:2000. Expected molecular weight: 35-43 kDa.
TBP antibody western blot analysis in (1) human HeLa, (2) (h) HepG2, (3) mouse NIH3T3 lysate. Expected molecular weight: 35-43 kDa.
Immunohistochemical analysis of paraffin-embedded human testis using TBP antibody at 1:25 dilution.
IHC testing of FFPE human testis tissue with TBP antibody at 1:25 dilution. HIER: pH6 citrate buffer.
IHC testing of FFPE mouse brain tissue with TBP antibody at 1:25 dilution. HIER: pH6 citrate buffer.
IHC testing of FFPE rat brain tissue with TBP antibody at 1:25 dilution. HIER: pH6 citrate buffer.
IHC testing of FFPE monkey brain tissue with TBP antibody at 1:25 dilution. HIER: pH6 citrate buffer.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat, Monkey
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name 830CT4.3.3
Purity Purified
UniProt P20226
Localization Nuclear
Applications IHC (Paraffin) : 1:25
Western blot : 1:1000-2:000
Limitations This TBP antibody is available for research use only.
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Description

General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.

Application Notes

Titration of the TBP antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Purified His-tagged protein was used to produced this monoclonal TBP antibody.

Storage

Aliquot the TBP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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