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Home >> Antibodies >> TAF12 Antibody

TAF12 Antibody (RQ4927)

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  Catalog No Formulation Size Price (USD)  
Image RQ4927 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of human 1) placenta, 2) U-937, 3) K562 and 4) ThP-1 lysate with TAF12 antibody at 0.5ug/ml. Expected molecular weight ~21 kDa.
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Western blot testing of human 1) placenta, 2) U-937, 3) K562 and 4) ThP-1 lysate with TAF12 antibody at 0.5ug/ml. Expected molecular weight ~21 kDa.
IHC staining of FFPE human lung cancer with TAF12 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE mouse brain with TAF12 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE rat brain with TAF12 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q16514
Localization Nuclear
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This TAF12 antibody is available for research use only.
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Description

Transcription initiation factor TFIID subunit 12 is a protein that in humans is encoded by the TAF12 gene. Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I. Two transcript variants encoding the same protein have been found for this gene.

Application Notes

Optimal dilution of the TAF12 antibody should be determined by the researcher.

Immunogen

Amino acids QIADDFIESVVTAACQLARHRKSSTLEVKDVQLHLERQ from the human protein were used as the immunogen for the TAF12 antibody.

Storage

After reconstitution, the TAF12 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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