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Home >> Antibodies >> Syndecan 4 Antibody

Syndecan 4 Antibody (R32064)

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  Catalog No Formulation Size Price (USD)  
Image R32064 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 419
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Western blot testing of 1) human MCF7, 2) human SW620 and 3) rat lung lysate with Syndecan 4 antibody. Expected/observed molecular weight: ~22 kDa.
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Western blot testing of 1) human MCF7, 2) human SW620 and 3) rat lung lysate with Syndecan 4 antibody. Expected/observed molecular weight: ~22 kDa.
IHC testing of FFPE human breast cancer with Syndecan 4 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE mouse intestine with Syndecan 4 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat intestine with Syndecan 4 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P31431
Localization Membrane
Applications Western blot : 0.1-0.5ug/ml
IHC (FFPE) : 0.5-1ug/ml
Limitations This Syndecan 4 antibody is available for research use only.
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Description

Syndecan 4 is also known as SDC4. The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein.

Application Notes

Optimal dilution of the Syndecan 4 antibody should be determined by the researcher.

Immunogen

Amino acids MKKKDEGSYDLGKKPIYKKAPTNEFYA of human Syndecan 4 were used as the immunogen for the Syndecan 4 antibody.

Storage

After reconstitution, the Syndecan 4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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