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Home >> Antibodies >> SUMO-2 Antibody

SUMO-2 Antibody [clone 973CT8.1.1] (F52344)

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  Catalog No Formulation Size Price (USD)  
Image F52344-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F52344-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Fluorescent image of HeLa cells stained with SUMO-2 antibody at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
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Fluorescent image of HeLa cells stained with SUMO-2 antibody at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
SUMO-2 antibody western blot analysis in (1) CEM, (2) 293, (3) rat C6 lysate. Observed molecular weight: 12-15 kDa.
Availability 1-3 business days
Species Reactivity Human, Rat
Predicted Reactivity Bovine, Chicken, Hamster, Mouse, Pig, Primate
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b
Clone Name 973CT8.1.1
Purity Purified
UniProt P61956
Applications Immunofluorescence : 1:25
Western blot : 1:1000
Limitations This SUMO-2 antibody is available for research use only.
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Description

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins.

Application Notes

Titration of the SUMO-2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Purified His-tagged protein was used to produced this monoclonal SUMO-2 antibody.

Storage

Aliquot the SUMO-2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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