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Home >> Antibodies >> Steroidogenic Factor 1 Antibody / SF-1 / R5A1

Steroidogenic Factor 1 Antibody / SF-1 / R5A1 [clone SF1/8000R] (V5306)

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  Catalog No Formulation Size Price (USD)  
Image V5306-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V5306-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V5306SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human adrenal cortex with Steroidogenic Factor 1 antibody (clone SF1/8000R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human adrenal cortex with Steroidogenic Factor 1 antibody (clone SF1/8000R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human testis tissue with Steroidogenic Factor 1 antibody (clone SF1/8000R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name SF1/8000R
Purity Protein A/G affinity
UniProt Q13285
Localization Nucleus
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Steroidogenic Factor 1 antibody is available for research use only.
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Description

Steroidogenic Factor 1 (SF-1) is considered an orphan nuclear receptor that belongs to subfamily 5. It was found to be a regulator of steroidogenic enzyme gene expression. Oxysterols are suggested as its ligands. It is expressed in all steroidogenic tissues, including the adrenal cortex, testicular Sertoli cells, and Leydig cells, ovarian theca, hypothalamus, and anterior pituitary. SF-1 plays an important role in adrenal and gonadal development.SF-1 is highly valuable marker to determine the adrenocortical origin of an adrenal mass.

Application Notes

Optimal dilution of the Steroidogenic Factor 1 antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 200-400) from the human protein was used as the immunogen for the Steroidogenic Factor 1 antibody.

Storage

Aliquot the Steroidogenic Factor 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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