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Home >> Antibodies >> StAR Antibody / Steroidogenic acute regulatory protein

StAR Antibody / Steroidogenic acute regulatory protein [clone STAR/3976] (V5238)

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  Catalog No Formulation Size Price (USD)  
Image V5238-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V5238-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V5238SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human adrenal gland tissue with StAR antibody (clone STAR/3976). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human adrenal gland tissue with StAR antibody (clone STAR/3976). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free StAR antibody (clone STAR/3976) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name STAR/3976
Purity Protein A/G affinity
UniProt P49675
Localization Cytoplasm (Mitochondria)
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This StAR antibody is available for research use only.
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Description

Steroidogenic Acute Regulatory Protein (STAR) controls the rate-limiting step of steroidegenesis by translocating cholesterol from the outer mitochondrial membrane to the inner membrane where it is later cleaved to pregnenolone. It is primarily present in steroid-producing cells, including Leydig cells in the testis, theca cells and luteal cells in the ovary and adrenal cells in the adrenal cortex. Due to low levels of pregnenolone, seminomas and Leydig cell tumors display no specific STAR staining. Therefore, STAR antibody may assist in differentiating sex cord stromal tumors (SCST), seminomas and embryonal carcinomas.

Application Notes

Optimal dilution of the StAR antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 39-108) from the human protein was used as the immunogen for the StAR antibody.

Storage

Aliquot the StAR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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