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Home >> Antibodies >> SSEA-1 Antibody Cocktail

SSEA-1 Antibody Cocktail [clone FUT4/815 + BRA-4F1] (V2524)

  Catalog No Formulation Size Price (USD)  
Image V2524-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V2524-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V2524SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V2524IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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IHC: Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with SSEA-1 antibody (FUT4/815 + BRA-4F1).
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgM, kappa
Clone Name FUT4/815 + BRA-4F1
Purity PEG precipitation
UniProt P22083
Localization Cytoplasmic and cell surface
Applications Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations This SSEA-1 antibody is available for research use only.
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Description

SSEA-1/CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, it is expressed in Reed-Sternberg cells and some epithelial cells. SSEA-1/CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.

Application Notes

Optimal dilution of the SSEA-1 antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Purified human neutrophils (FUT4/815) and Myelomonocytic leukemia cells (BRA-4F1) were used as the immunogen for the SSEA-1 antibody cocktail.

Storage

Store the SSEA-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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