SP1 Antibody | Specific Protein 1 | RQ4532 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	RQ4532	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	429
Formulation

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IHC staining of FFPE human esophagus squama cancer with SP1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

IHC staining of FFPE human ovarian cancer with SP1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

IHC staining of FFPE human intestinal cancer with SP1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

IHC staining of FFPE human lung cancer with SP1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human 1) K562, 2) A549, 3) HeLa, 4) A431, 5) rat brain and 6) mouse brain lysate with SP1 antibody. Expected molecular weight: 81-95 kDa.

IHC staining of FFPE human tonsil with SP1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

IHC staining of FFPE mouse liver with SP1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

IHC staining of FFPE rat liver with SP1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

ICC staining of FFPE human HeLa cells with SP1 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of FFPE human A549 cells with SP1 antibody (green) at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.

Availability	1-3 business days
Species Reactivity	Human, Mouse, Rat
Format	Antigen affinity purified
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Antigen affinity
Buffer	Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt	P08047
Localization	Nuclear, cytoplasmic
Applications	Western blot : 0.1-0.5ug/ml IHC (FFPE) : 1-2ug/ml Immunofluorescence/Immunocytochemistry : 2-4ug/ml Immunocytochemistry : 1-2ug/ml Direct ELISA : 0.1-0.5ug/ml (human recombinant protein)
Limitations	This SP1 antibody is available for research use only.
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Description

Transcription factor Sp1, also known as 'Specificity protein 1' is a protein that in humans is encoded by the SP1 gene. The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene.

Application Notes

Optimal dilution of the SP1 antibody should be determined by the researcher.

Immunogen

Amino acids 384-603 of the human protein were used as the immunogen for the SP1 antibody.

Storage

After reconstitution, the SP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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SP1 Antibody / Specific Protein 1 (RQ4532)

Description

Application Notes

Immunogen

Storage

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Area of Research

Product Type

Protocols

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