SORL1 Antibody / Sortilin-related receptor / SORLA (FY12181)

SORL1 Antibody Human Breast Cancer IHC. Immunohistochemical staining of FFPE human breast cancer tissue with SORL1 antibody demonstrated moderate cytoplasmic and membranous staining in malignant epithelial cells. SORL1 (Sortilin-Related Receptor 1) is a VPS10-domain sorting receptor that regulates intracellular protein trafficking, endosomal sorting, and receptor recycling to maintain cellular protein homeostasis. Altered SORL1 expression has been associated with neurodegenerative disease and several human cancers. These results support the utility of SORL1 Antibody for studies of intracellular trafficking, endosomal biology, receptor sorting, and cancer. Heat-mediated antigen retrieval was performed in pH 8.0 EDTA buffer, followed by overnight incubation with SORL1 antibody at 2 ug/ml. Detection was achieved using an HRP-conjugated secondary antibody and DAB substrate.

SORL1 Antibody Rat and Mouse Brain WB. Western blot analysis of SORL1 expression was performed using anti-SORL1 antibody. Lane 1: rat brain tissue lysate. Lane 2: mouse brain tissue lysate. SORL1 (Sortilin-Related Receptor 1) is a VPS10-domain sorting receptor that regulates intracellular protein trafficking, endosomal sorting, and receptor recycling, particularly within neurons where it controls amyloid precursor protein (APP) trafficking. A specific immunoreactive band is detected at approximately 250 kDa, consistent with the predicted molecular weight of SORL1 (~248 kDa). These results demonstrate cross-species reactivity and support the utility of SORL1 Antibody for studies of neuronal protein trafficking, endosomal biology, neurodegeneration, and Alzheimer's disease research.

SORL1 Antibody Mouse Brain IHC. Immunohistochemical staining of FFPE mouse brain tissue with SORL1 antibody demonstrated moderate cytoplasmic and membranous staining in neuronal cells, consistent with the localization of SORL1 (Sortilin-Related Receptor 1), a VPS10-domain sorting receptor that regulates intracellular protein trafficking, endosomal sorting, and receptor recycling. SORL1 plays a critical role in neuronal protein homeostasis by directing amyloid precursor protein (APP) trafficking, making it an important marker in Alzheimer's disease and neurodegeneration research. Heat-mediated antigen retrieval was performed in pH 8.0 EDTA buffer, followed by overnight incubation with SORL1 antibody at 2 ug/ml. Detection was achieved using an HRP-conjugated secondary antibody and DAB substrate.

SORL1 Antibody Rat Brain IHC. Immunohistochemical staining of FFPE rat brain tissue with SORL1 antibody demonstrated moderate cytoplasmic and membranous staining in neuronal cells, consistent with the localization of SORL1 (Sortilin-Related Receptor 1), a VPS10-domain sorting receptor that regulates intracellular protein trafficking, endosomal sorting, and receptor recycling. SORL1 is a key regulator of amyloid precursor protein (APP) trafficking and neuronal protein homeostasis, making it an important marker in Alzheimer's disease and neurodegeneration research. Heat-mediated antigen retrieval was performed in pH 8.0 EDTA buffer, followed by overnight incubation with SORL1 antibody at 2 ug/ml. Detection was achieved using an HRP-conjugated secondary antibody and DAB substrate.

SORL1 Antibody Human Breast Cancer IF. Immunofluorescent staining of FFPE human breast cancer tissue with SORL1 antibody (red) demonstrated prominent cytoplasmic and membranous localization in malignant epithelial cells. SORL1 (Sortilin-Related Receptor 1) is a VPS10-domain sorting receptor that regulates intracellular protein trafficking, endosomal sorting, and receptor recycling, thereby maintaining cellular protein homeostasis. Altered SORL1 expression has been implicated in neurodegenerative disorders and several human cancers. Nuclei were counterstained with DAPI (blue). Heat-mediated antigen retrieval was performed in pH 8.0 EDTA buffer, followed by overnight incubation with SORL1 antibody at 5 ug/ml and detection using Cy3-conjugated goat anti-rabbit IgG.

SORL1 Antibody Human U251 Flow Cytometry. Flow cytometric analysis of human U251 cells using SORL1 antibody demonstrated a clear rightward shift in fluorescence intensity (blue) compared with the isotype control (green) and unstained cells (red), indicating specific detection of SORL1 expression. SORL1 (Sortilin-Related Receptor 1) is a VPS10-domain sorting receptor that regulates intracellular protein trafficking, endosomal sorting, and receptor recycling. U251 cells were fixed with 4% paraformaldehyde, blocked with normal goat serum, and incubated with SORL1 antibody at 1 ug/million cells, followed by DyLight 488-conjugated secondary antibody detection. These results support the utility of SORL1 Antibody for flow cytometric studies of intracellular trafficking, receptor sorting, and neurobiology.