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Home >> Antibodies >> SOD1 Antibody

SOD1 Antibody (R31855)

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Image R31855 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 419
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Western blot testing of rat 1) brain, 2) spleen, 3) liver, 4) kidney and mouse 5) brain, 6) spleen, 7) liver, 8) kidney and 9) HEPA1-6 lysate with SOD1 antibody. Predicted molecular weight ~16 kDa.
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Western blot testing of rat 1) brain, 2) spleen, 3) liver, 4) kidney and mouse 5) brain, 6) spleen, 7) liver, 8) kidney and 9) HEPA1-6 lysate with SOD1 antibody. Predicted molecular weight ~16 kDa.
Western blot testing of human 1) placenta, 2) HeLa, 3) MDA-MB-231, 4) SW620, 5) A549, 6) HepG2, 7) Jurkat and 8) K562 lysate with SOD1 antibody. Predicted molecular weight ~16 kDa.
IF/ICC staining of FFPE human Hep2 cells with SOD1 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human A549 cells with SOD1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SOD1 antibody.
IHC staining of FFPE human intestinal cancer with SOD1 antibody. HIER: boil tissue sections in pH 8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human intestinal cancer with SOD1 antibody. HIER: boil tissue sections in pH 8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with SOD1 antibody. HIER: boil tissue sections in pH 8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with SOD1 antibody. HIER: boil tissue sections in pH 8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with SOD1 antibody. HIER: boil tissue sections in pH 8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with SOD1 antibody. HIER: boil tissue sections in pH 8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse liver with SOD1 antibody. HIER: boil tissue sections in pH 8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat liver with SOD1 antibody. HIER: boil tissue sections in pH 8 EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P00441
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Flow cytometry : 1-3ug/10^6 cells
Immunofluorescence/Immunocytochemistry : 2-4ug/ml
Limitations This SOD1 antibody is available for research use only.
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Description

Superoxide dismutases (SOD) are a class of enzymes that catalyze the dismutation of superoxide into oxygen and hydrogen peroxide. As such, they are an important antioxidant defense in nearly all cells exposed to oxygen. One of the exceedingly rare exceptions is Lactobacillus plantarum and related lactobacilli, which use a different mechanism. Cu,Zn-SOD was found widely distributed in the cell cytosol and in the cell nucleus, consistent with it being a soluble cytosolic protein. Mitochondria and secretory compartments did not label for this protein. In human cells, peroxisomes showed a labeling density slightly less than that of cytoplasm.

Application Notes

Optimal dilution of the SOD1 antibody should be determined by the researcher.

Immunogen

Amino acids RTLVVHEKADDLGKGGNEESTKTGNAGSRLA of human SOD1 were used as the immunogen for the SOD1 antibody.

Storage

After reconstitution, the SOD1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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