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Home >> Antibodies >> Smooth Muscle Actin Antibody

Smooth Muscle Actin Antibody [clone 1A4] (V2001)

  Catalog No Formulation Size Price (USD)  
V2001-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 329

V2001-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 129

V2001SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 359

V2001IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 329
IHC: human leiomyosarcoma stained with Smooth Muscle Actin antibody (1A4).
Western blot testing of human uterus lysate with Smooth Muscle Actin antibody (clone 1A4).
Species Reactivity Human, Baboon, Monkey, Cow, Pig, Sheep, Goat, Cat, Dog, Rabbit, Mouse, Rat, Guinea Pig and Chicken.
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name 1A4
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
Gene ID 59
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT (1)
Prediluted IHC only format : incubate for 30 min at RT (2)
Limitations This Smooth Muscle Actin antibody is available for research use only.

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Description

Actin is a major component of the cytoskeleton and is present in most cell types. This Smooth muscle actin antibody is highly specific to the smooth muscle form. Its epitope lies in the first four N-terminal amino acids. The Smooth muscle actin antibody does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. It could be used together with antimuscle specific actin and myogenin in making a diagnosis of smooth/skeletal muscle tumors. In most cases of rhabdomyosarcoma, this smooth form antibody yields negative results whereas antimuscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with antimuscle specific actin and are negative with anti-myogenin.

Application Notes

Variations in protocols, secondaries and substrates may require the Smooth Muscle Actin antibody to be titered up or down for optimal performance.

1. No special pretreatment is required for the immunohistochemical staining of FFPE tissue.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

An N-Terminal decapeptide of the alpha smooth muscle isoform was used as the immunogen for this Smooth Muscle Actin antibody.

Storage

Store the Smooth Muscle Actin antibody at 2-8°C (aliquot/freeze the azide-free format).

Alternate Names

ACTA2, Smooth form antibody

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