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Home >> Antibodies >> SMAD2 Antibody

SMAD2 Antibody (F52960)

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  Catalog No Formulation Size Price (USD)  
Image F52960-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F52960-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of lysate from (1) HeLa, (2) mouse NIH3T3, (3) mouse C2C12, and (4) rat PC-12 cell line using SMAD2 antibody at 1:1000. Predicted molecular weight: 52~60 kDa.
Western blot analysis of lysate from (1) HeLa, (2) mouse NIH3T3, (3) mouse C2C12, and (4) rat PC-12 cell line using SMAD2 antibody at 1:1000. Predicted molecular weight: 52~60 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Predicted Reactivity Bovine, Pig, Chicken
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt Q62432
Applications Western blot : 1:500-1:1000
Limitations This SMAD2 antibody is available for research use only.
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Description

SMAD2 is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. [UniProt]

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the SMAD2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This SMAD2 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 198-232 amino acids of mouse Smad2.

Storage

Store at 4oC for up to one month. For long term, aliquot the SMAD2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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