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Home >> Antibodies >> SI-CLP Antibody / CHID1

SI-CLP Antibody / CHID1 [clone CHID1/6368] (V4152)

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  Catalog No Formulation Size Price (USD)  
Image V4152-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4152-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4152SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human prostate tissue with SI-CLP antibody (clone CHID1/6368) HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human prostate tissue with SI-CLP antibody (clone CHID1/6368) HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human esophagus tissue with SI-CLP antibody (clone CHID1/6368) Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name CHID1/6368
Purity Protein A/G affinity
UniProt Q9BWS9
Localization Secreted, Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This SI-CLP antibody is available for research use only.
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Description

SI-CLP (stabilin-1 interacting chitinase-like), also known as CHID1 (chitinase domain-containing protein 1), is a 393 amino acid secreted protein that belongs to the glycosyl hydrolase 18 family. SI-CLP interacts with STAB1 and localizes to lysosome in cells of monocytic, T, B and epithelial origin. SI-CLP is a saccharide- and LPS-binding protein with possible roles in pathogen sensing and endotoxin neutralization. Ligand-binding specificity relates to the length of the oligosaccharides, with preference for chitotetraose (in vitro). The SI-CLP protein is up-regulated by IL4/interleukin-4 and dexamethasone in the macrophages, and is also up-regulated by glucocorticoid. Existing as three alternatively spliced isoforms, the SI-CLP gene is conserved in chimpanzee, canine, bovine, mouse, rat, chicken, zebrafish, fruit fly, mosquito, A. thaliana and rice, and maps to human chromosome 11p15.5.

Application Notes

Optimal dilution of the SI-CLP antibody should be determined by the researcher.

Immunogen

Recombinant full-length human SI-CLP protein was used as the immunogen for the CHID1 antibody.

Storage

Aliquot the SI-CLP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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