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Home >> Antibodies >> Septin 2 Antibody / SEPT2

Septin 2 Antibody / SEPT2 (RQ7247)

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  Catalog No Formulation Size Price (USD)  
Image RQ7247 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE mouse brain tissue with Septin 2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
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Immunofluorescent staining of FFPE mouse brain tissue with Septin 2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE rat brain tissue with Septin 2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human endometrial cancer tissue with Septin 2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human SiHa cells with Septin 2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE mouse brain tissue with Septin 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with Septin 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with Septin 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with Septin 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human MCF7, 2) human U-87 MG, 3) human HeLa, 4) human K562, 5) monkey COS-7, 6) human HepG2 and 7) human HEL cell lysate with Septin 2 antibody. Predicted molecular weight: ~41 kDa.
Western blot testing of 1) rat brain, 2) rat kidney, 3) rat testis, 4) rat C6, 5) mouse brain, 6) mouse testis and 7) mouse Neuro-2a cell lysate with Septin 2 antibody. Predicted molecular weight: ~41 kDa.
Flow cytometry testing of human U-251 cells with Septin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Septin 2 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat, Monkey
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q15019
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Septin 2 antibody is available for research use only.
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  • Applications : WB, IHC-P, ELISA (peptide)
    Reactivity : Human
    Pred. Reactivity : Mouse, Rat, Cow

Description

Septin 2, also known as SEPT2, is a protein which in humans is encoded by the SEPT2 gene. Enables identical protein binding activity. Predicted to be involved in several processes, including cilium assembly; regulation of exocytosis; and smoothened signaling pathway. Predicted to act upstream of or within regulation of L-glutamate import across plasma membrane and regulation of protein localization. Located in several cellular components, including cytoskeleton; photoreceptor connecting cilium; and sperm annulus. Part of septin complex.

Application Notes

Optimal dilution of the Septin 2 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids M1-E326) was used as the immunogen for the Septin 2 antibody.

Storage

After reconstitution, the Septin 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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