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Home >> Antibodies >> SBDS Antibody / Shwachman-Bodian-Diamond syndrome protein

SBDS Antibody / Shwachman-Bodian-Diamond syndrome protein (RQ7423)

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  Catalog No Formulation Size Price (USD)  
Image RQ7423 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human HL60 cells with SBDS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SBDS antibody.
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Flow cytometry testing of human HL60 cells with SBDS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SBDS antibody.
Flow cytometry testing of human Caco-2 cells with SBDS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SBDS antibody.
Western blot testing of human 1) 293T, 2) HepG2 and 3) HL60 cell lysate with SBDS antibody. Predicted molecular weight ~29 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9Y3A5
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This SBDS antibody is available for research use only.
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Description

Ribosome maturation protein SBDS is a protein that in humans is encoded by the SBDS gene. This gene encodes a highly conserved protein that plays an essential role in ribosome biogenesis. The encoded protein interacts with elongation factor-like GTPase 1 to disassociate eukaryotic initiation factor 6 from the late cytoplasmic pre-60S ribosomal subunit allowing assembly of the 80S subunit. Mutations within this gene are associated with the autosomal recessive disorder Shwachman-Bodian-Diamond syndrome. This gene has a closely linked pseudogene that is distally located.

Application Notes

Optimal dilution of the SBDS antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids R19-D241) was used as the immunogen for the SBDS antibody.

Storage

After reconstitution, the SBDS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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