SART1 Antibody (RQ7452)

Immunofluorescent staining of FFPE human A549 cells with SART1 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.

Immunofluorescent staining of FFPE human breast cancer tissue with SART1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

IHC staining of FFPE human colorectal adenocarcinoma tissue with SART1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lung cancer tissue with SART1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with SART1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human ovarian cancer tissue with SART1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human breast cancer tissue with SART1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of 1) human HeLa, 2) human MCF7, 3) human MOLT-4, 4) rat testis, 5) mouse testis and 6) mouse thymus tissue lysate with SART1 antibody. Predicted molecular weight: ~90 kDa, routinely observed at 110-125 kDa.

Flow cytometry testing of human U-2 OS cells with SART1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SART1 antibody.

Flow cytometry testing of human JK-1 cells with SART1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SART1 antibody.