Immunofluorescent staining of FFPE rat brain tissue with SAM68 antibody (red). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE mouse brain tissue with SAM68 antibody (red). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human intestinal cancer tissue with SAM68 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human A549 cells with SAM68 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human papillary thyroid carcinoma tissue with SAM68 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian adenocarcinoma tissue with SAM68 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human keratinizing squamous cell carcinoma tissue with SAM68 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with SAM68 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with SAM68 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human glioblastoma tissue with SAM68 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with SAM68 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with SAM68 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) human 293T, 3) human Raji, 4) human Daudi, 5) rat brain, 6) rat PC-12, 7) mouse brain and 8) mouse NIH 3T3 cell lysate with SAM68 antibody. Predicted molecular weight 48 kDa but routinely observed at 62-68 kDa.
Flow cytometry testing of fixed and permeabilized human Raji cells with SAM68 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SAM68 antibody.
Flow cytometry testing of fixed and permeabilized mouse HEPA1-6 cells with SAM68 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SAM68 antibody.