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Home >> Antibodies >> S100A5 Antibody / S100 calcium binding protein A5

S100A5 Antibody / S100 calcium binding protein A5 [clone S100A5/7472] (V4727)

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  Catalog No Formulation Size Price (USD)  
Image V4727-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4727-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4727SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human brain tissue with S100A5 antibody (clone S100A5/7472). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human brain tissue with S100A5 antibody (clone S100A5/7472). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free S100A5 antibody (clone S100A5/7472) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name S100A5/7472
Purity Protein A/G affinity
UniProt P33763
Localization Nucleus, Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This S100A5 antibody is available for research use only.
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Description

S-100A5 (S100 calcium binding protein A5) is a 92 amino acid protein that belongs to the S-100 family. Containing two EF-hand domains, S-100A5 binds calcium, zinc and copper. One subunit can simultaneously bind two calcium ions or two copper ions plus one zinc ion. Calcium and copper ions compete for the same binding sites on the S-100A5 protein.

Application Notes

Optimal dilution of the S100A5 antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 1-92) from the human protein was used as the immunogen for the S100A5 antibody.

Storage

Aliquot the S100A5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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