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Home >> Antibodies >> S100 beta Antibody [Discontinued, see V3429]

S100 beta Antibody [Discontinued, see V3429] [clone SH-B1, aka SA-12] (R30077)

  Catalog No Formulation Size Price (USD)  
R30077 0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS 100 ug 275
IHC-P: S100 beta antibody testing of rat brain tissue lysate. HIER: steamed with pH6 citrate buffer.
Availability Discontinued
Species Reactivity Human, rabbit, rat
Format Ascites
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name SH-B1, aka SA-12
Purity Ascites
Gene ID 6285
Applications IHC (Paraffin) : 1-2ug/ml
Limitations This S100 beta antibody is available for research use only.

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Description

S100 protein is a 21,000-Da component first isolated from brain by Moore(1965). Immunocytochemical studies demonstrated that S100 protein is produced by a wide variety of normal and neoplastic cells of mesodermal, neuroectodermal, and epithelial origin(Herrera et al., 1988). The S100 molecule is a dimer; thus there are 3 forms of S100: alpha-alpha, known as S-100a(0); alpha-beta, known as S-100a; and beta-beta, known as S-100b. S100 genes are clustered on human chromosome 1q21. S-100 protein expression by primary and metastatic adenocarcinomas.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the S100 beta antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Bovine brain S-100b was used as the immunogen for this S100 beta antibody.

Storage

After reconstitution, the S100 beta antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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