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Home >> Antibodies >> Recombinant Vimentin Antibody / C-Terminal

Recombinant Vimentin Antibody / C-Terminal [clone RM289] (R20315)

  Catalog No Formulation Size Price (USD)  
Image R20315-0.1ML Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ul 419
Microvalidated Recrabbitmono
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Western blot testing of human HeLa lysate with recombinant Vimentin antibody at 1:400 dilution. Predicted molecular weight ~53 kDa.
IHC testing of formalin fixed and paraffin embedded human kidney tissue with recombinant Vimentin antibody at 1:200 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM289
Purity Protein A purified from animal origin-free supernatant
UniProt P08670
Localization Cytoplasmic, cell membrane
Applications Immunohistochemistry (FFPE) : 1:100-1:200
Western Blot : 1:200-1:500
Limitations This recombinant Vimentin antibody is available for research use only.
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Description

This antibody reacts to human Vimentin. It may also react to mouse or rat Vimentin, as predicted by immunogen homology.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Vimentin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A peptide corresponding to the C-terminus of human Vimentin was used as the immunogen for the recombinant Vimentin antibody.

Storage

Store the recombinant Vimentin antibody at -20oC.

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