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Home >> Antibodies >> Recombinant VIM Antibody / Vimentin

Recombinant VIM Antibody / Vimentin [clone rVIM/6914] (V9616)

  Catalog No Formulation Size Price (USD)  
Image V9616-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9616-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9616SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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Western blot testing of human U-87 MG cell lysate using recombinant VIM antibody (clone rVIM/6914). Predicted molecular weight ~53 kDa.
IHC staining of FFPE human tonsil tissue with recombinant VIM antibody (clone rVIM/6914). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant VIM antibody (clone rVIM/6914) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rVIM/6914
Purity Protein A/G affinity
UniProt P08670
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Western blot : 1-2ug/ml
Limitations This recombinant VIM antibody is available for research use only.
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Description

When used in conjunction with anti-keratin staining, anti-vimentin staining is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma.

Application Notes

Optimal dilution of the recombinant VIM antibody should be determined by the researcher.

Immunogen

A portion of amino acids 366-466 was used as the immunogen for the recombinant VIM antibody.

Storage

Aliquot the recombinant VIM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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