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Home >> Antibodies >> Recombinant Tyrosinase Antibody

Recombinant Tyrosinase Antibody [clone TYR/2024R] (V3657)

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  Catalog No Formulation Size Price (USD)  
Image V3657-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3657-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3657SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3657IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recrabbitmono
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IHC: Formalin-fixed, paraffin-embedded human melanoma stained with recombinant Tyrosinase antibody (clone TYR/2024R). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, or 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
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IHC: Formalin-fixed, paraffin-embedded human melanoma stained with recombinant Tyrosinase antibody (clone TYR/2024R). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, or 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant Tyrosinase antibody (clone TYR/2024R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name TYR/2024R
Purity Protein A affinity chromatography
UniProt P14679
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This recombinant Tyrosinase antibody is available for research use only.
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Description

Recognizes a cluster of proteins between 70-80kDa, identified as tyrosinase. Occasionally a minor band at 55kDa is also detected. This mAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this mAb shows tyrosinase in melanotic as well as amelanotic variants. This mAb is a useful marker for melanocytes and melanomas.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Tyrosinase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant protein was used as the immunogen for this recombinant Tyrosinase antibody.

Storage

Store the recombinant Tyrosinase antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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