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Home >> Antibodies >> Recombinant Topoisomerase II alpha Antibody / TOP2A

Recombinant Topoisomerase II alpha Antibody / TOP2A [clone TOP2A/6570R] (V9310)

  Catalog No Formulation Size Price (USD)  
Image V9310-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9310-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9310SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human lymph node tissue with recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue using recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human HeLa and Jurkat cell lysates using recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R). Predicted molecular weight ~174 kDa.
SDS-PAGE analysis of purified, BSA-free recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name TOP2A/6570R
Purity Protein A/G affinity
UniProt P11388
Localization Nuclear
Applications Western blot : 2-4ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant Topoisomerase II alpha antibody is available for research use only.
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Description

The topoisomerase IIa is the predominant mechanism of resistance to several chemotherapeutic agents.

Application Notes

Optimal dilution of the recombinant Topoisomerase II alpha antibody should be determined by the researcher.

Immunogen

A portion of amino acids 1352-1493 was used as the immunogen for the recombinant Topoisomerase II alpha antibody.

Storage

Aliquot the recombinant Topoisomerase II alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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