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Home >> Antibodies >> Recombinant Topoisomerase II alpha Antibody / TOP2A

Recombinant Topoisomerase II alpha Antibody / TOP2A [clone rTOP2A/6629] (V8971)

  Catalog No Formulation Size Price (USD)  
Image V8971-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V8971-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V8971SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human lymph node with recombinant Topoisomerase II alpha antibody (clone rTOP2A/6629). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant Topoisomerase II alpha antibody (clone rTOP2A/6629). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Topoisomerase II alpha antibody (clone rTOP2A/6629) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rTOP2A/6629
Purity Protein A/G affinity
UniProt P11388
Localization Nuclear
Applications Immunohistochemistry (FFPE) : 0.5-1ug/ml
Limitations This recombinant Topoisomerase II alpha antibody is available for research use only.
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Description

Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and re-ligating the broken strand. [UniProt]

Application Notes

Optimal dilution of the recombinant Topoisomerase II alpha antibody should be determined by the researcher.

Immunogen

A portion of amino acids 1352-1493 was used as the immunogen for the recombinant Topoisomerase II alpha antibody.

Storage

Aliquot the recombinant Topoisomerase II alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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