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Home >> Antibodies >> Recombinant TOP2A Antibody / Topoisomerase II alpha

Recombinant TOP2A Antibody / Topoisomerase II alpha [clone rTOP2A/6569] (V9302)

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  Catalog No Formulation Size Price (USD)  
Image V9302-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9302-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9302SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human lymph node with recombinant TOP2A antibody (clone rTOP2A/6569). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human lymph node with recombinant TOP2A antibody (clone rTOP2A/6569). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant TOP2A antibody (clone rTOP2A/6569). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue with recombinant TOP2A antibody (clone rTOP2A/6569) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa and 2) Jurkat cell lysate using recombinant TOP2A antibody (clone rTOP2A/6569). Predicted molecular weight ~174 kDa.
SDS-PAGE analysis of purified, BSA-free recombinant TTOP2A antibody (clone rTOP2A/6569) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG2a, kappa
Clone Name rTOP2A/6569
Purity Protein A/G affinity
UniProt P11388
Localization Nuclear
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml
Limitations This recombinant TOP2A antibody is available for research use only.
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Description

It recognizes a 170kDa protein, which is identified as topoisomerase II is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents.

Application Notes

Optimal dilution of the recombinant TOP2A antibody should be determined by the researcher.

Immunogen

A portion of amino acids 1352-1493 was used as the immunogen for the recombinant TOP2A antibody.

Storage

Aliquot the recombinant TOP2A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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