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Home >> Antibodies >> Recombinant TOP2A Antibody / Topoisomerase II alpha

Recombinant TOP2A Antibody / Topoisomerase II alpha [clone rTOP2A/8339] (V4483)

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  Catalog No Formulation Size Price (USD)  
Image V4483-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4483-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4483SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human tonsil tissue with recombinant TOP2A antibody (clone rTOP2A/8339). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant TOP2A antibody (clone rTOP2A/8339). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rTOP2A/8339
Purity Protein A/G affinity
UniProt P11388
Localization Nucleus
Applications Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 minutes at RT
Limitations This recombinant TOP2A antibody is available for research use only.
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Description

It recognizes a 170kDa protein, which is identified as topoisomerase IIAlpha. It shows no cross-reaction with Topoisomerase II beta or I. Topo II Alpha plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo II Alpha is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents.

Application Notes

Optimal dilution of the recombinant TOP2A antibody should be determined by the researcher.

Immunogen

A recombinant human Topoisomerase II alpha protein fragment (aa1300-1500) was used as the immunogen for the recombinant TOP2A antibody.

Storage

Aliquot the recombinant TOP2A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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