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Home >> Antibodies >> Recombinant Smac Antibody

Recombinant Smac Antibody [clone RM271] (R20288)

  Catalog No Formulation Size Price (USD)  
Image R20288-0.1ML Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ul 419
Microvalidated Recrabbitmono
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Western blot of human HeLa and MCF7 cell lysate using recombinant Smac antibody at 1:1000. Predicted molecular weight: 21~27 kDa.
IHC testing of FFPE human breast cancer tissue with recombinant Smac antibody at 1:1000.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM271
Purity Protein A purified from animal origin-free supernatant
UniProt Q9NR28
Gene ID 56616
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1:500-1:1000 (1)
Western blot : 1:1000-1:2000
Limitations This recombinant Smac antibody is available for research use only.
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Description

This recombinant Smac antibody reacts to human Smac (Diablo homolog, mitochondrial).

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Smac antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

A peptide corresponding to the C-terminus of human Smac/Diablo was used as the immunogen for this recombinant Smac antibody.

Storage

Store the recombinant Smac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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