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Home >> Antibodies >> Recombinant Rabbit IgG Fab Antibody (Biotin Conjugate)

Recombinant Rabbit IgG Fab Antibody (Biotin Conjugate) [clone RMG01] (R20172BTN)

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Image R20172BTN-50UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 50 ug 409
Microvalidated Recgoatmono
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ELISA of IgGs from different species shows the recombinant Rabbit IgG Fab antibody reacts to the Fab region of rabbit IgG; no cross reactivity with human IgG, rat IgG, or mouse IgG. The plate was coated with 50 ng/well of different IgG. 500 ng/mL, 200 ng/mL, or 50 ng/mL of RMG01 was used as the primary and an alkaline phosphatase conjugated anti-goat IgG as the secondary.
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ELISA of IgGs from different species shows the recombinant Rabbit IgG Fab antibody reacts to the Fab region of rabbit IgG; no cross reactivity with human IgG, rat IgG, or mouse IgG.
Western blot of nonreduced(-) and reduced(+) rabbit IgG (20ng/lane), using 0.2ug/ml of recombinant Rabbit IgG Fab antibody. This antibody reacts to nonreduced rabbit IgG (~150 kDa).
ELISA Titration: the plate was coated with different amounts of rabbit IgG. A serial dilution of the recombinant Rabbit IgG Fab antibody was used as the primary and an alkaline phosphatase conjugated anti-goat IgG as the secondary.
Availability 1-3 business days
Species Reactivity Rabbit
Format Biotin Conjugate
Clonality Recombinant Goat Monoclonal
Isotype Goat IgG
Clone Name RMG01
Purity Protein G purified from animal origin-free supernatant
Gene ID N/A
Applications ELISA : 0.01ug/ml-0.5ug/ml
Western Blot (non-reduced) : 0.1-0.5ug/ml
Limitations This recombinant Rabbit IgG Fab antibody is available for research use only.
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Description

This recombinant Rabbit IgG Fab antibody reacts to the Fab region of Rabbit IgG. No cross reactivity with human IgG, rat IgG, or mouse IgG

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Rabbit IgG Fab antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Rabbit IgG was used as the immunogen for this recombinant Rabbit IgG Fab antibody.

Storage

Store the recombinant Rabbit IgG Fab antibody at -20oC.

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