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Home >> Antibodies >> Recombinant PSAP Antibody

Recombinant PSAP Antibody [clone rACPP/1338] (V3536)

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  Catalog No Formulation Size Price (USD)  
Image V3536-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3536-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3536SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3536IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recmousemono
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IHC testing of human prostate carcinoma with recombinant PSAP antibody (clone rACPP/1338). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of human prostate carcinoma with recombinant PSAP antibody (clone rACPP/1338). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant PSAP antibody (clone rACPP/1338) as confirmation of integrity and purity.
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rACPP/1338
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
UniProt P15309
Gene ID 55
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations This recombinant PSAP antibody is available for research use only.
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Description

Prostate specific acid phosphatase (PSAP) is an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. PSAP is found in non-neoplastic adult and fetal prostatic glands, primary and metastatic prostatic carcinomas. It shows no staining in granulocytes, osteoclasts, parietal cells of the stomach, liver cells, renal cell or breast carcinomas.

Application Notes

Titering of the recombinant PSAP antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Human recombinant full length protein was used as the immunogen for this recombinant PSAP antibody.

Storage

The recombinant PSAP antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

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