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Home >> Antibodies >> Recombinant phospho-Stat3 Antibody (Tyr705)

Recombinant phospho-Stat3 Antibody (Tyr705) [clone RM261] (R20279)

  Catalog No Formulation Size Price (USD)  
Image R20279-0.1ML Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ul 419
Microvalidated Recrabbitmono
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Western blot of A431 cell lysate, untreated (-) or treated (+) with EGF, using recombinant phospho-Stat3 antibody at 1:1000.
IHC testing of FFPE human breast cancer tissue with recombinant phospho-Stat3 antibody at 1:10,000.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse, Rat
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM261
Purity Protein A purified from animal origin-free supernatant
UniProt P40763
Gene ID 6774
Applications Immunohistochemistry (FFPE) : 1:1000-1:10;000 (1)
Western blot : 1:1000-1:2000
Limitations This recombinant phospho-Stat3 antibody is available for research use only.
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Description

This recombinant phospho-Stat3 antibody reacts to human Stat3 only when phosphorylated at Tyr705. There is no cross-reactivity to Stat3 without phosphorylation at Tyr705. It may also react to mouse or rat phospho-Stat3 (Tyr705), as predicted by immunogen homology.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant phospho-Stat3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

A phospho-peptide corresponding to human phospho-Stat3 (Tyr705) was used as the immunogen for this recombinant phospho-Stat3 antibody.

Storage

Store the recombinant phospho-Stat3 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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