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Home >> Antibodies >> Recombinant phospho-Histone H2B Antibody (Ser14)

Recombinant phospho-Histone H2B Antibody (Ser14) [clone RM238] (R20243)

  Catalog No Formulation Size Price (USD)  
Image R20243-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 469
Image
R20243-25UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 25 ug 239
Microvalidated Recrabbitmono
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Western blot test of acid extracts of HeLa cells treated or non-treated with Nocodazole, using recombinant phospho-Histone H2B antibody at 0.5 ug/ml, showed a band of Histone H2B phosphorylated at Serine 14 in HeLa cells.
ICC/IF of HeLa cells using recombinant phospho-Histone H2B antibody (red) and DAPI (blue). Actin filaments have been labeled with fluorescein phalloidin (green).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM238
Purity Protein A purified from animal origin-free supernatant
UniProt P33778
Gene ID 3018
Applications Western blot : 0.5-2ug/ml
Immunocytochemistry : 1-2ug/ml
ELISA : 0.2-1ug/ml
Limitations This recombinant phospho-Histone H2B antibody is available for research use only.
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Description

This recombinant phospho-Histone H2B antibody reacts to Histone H2B only when phosphorylated at serine 14. No cross reactivity with other phosphorylated histones.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant phospho-Histone H2B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A phospho-peptide corresponding to phospho-Histone H2B (Ser14) was used as the immunogen for this recombinant phospho-Histone H2B antibody.

Storage

Store the recombinant phospho-Histone H2B antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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