- Tel: 858.663.9055
- Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
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This recombinant phospho-AKT antibody reacts to AKT1/2/3 only when phosphorylated at Ser473. There is no cross-reactivity with Akt without phosphorylation at Ser473. It may also react to bovine, mouse or rat phospho-AKT (Ser473), as predicted by immunogen homology
The stated application concentrations are suggested starting points. Titration of the recombinant phospho-AKT antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
A phospho-peptide corresponding to human phospho-Akt (Ser473) was used as the immunogen for this recombinant phospho-AKT antibody.
Store the recombinant phospho-AKT antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
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