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Home >> Antibodies >> Recombinant Paxillin Antibody

Recombinant Paxillin Antibody [clone RM256] (R20277)

  Catalog No Formulation Size Price (USD)  
Image R20277-0.1ML Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ul 419
Microvalidated Recrabbitmono
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Western blot testing of human HeLa cell lysate with recombinant Paxillin antibody at 1:1000. Predicted molecular weight: 61/65/66 kDa (alpha/beta/gamma).
ICC/IF staining of HeLa cells using recombinant Paxillin antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nucleus labeled with DAPI (blue).
IHC testing of FFPE human tonsil tissue with recombinant Paxillin antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse, Rat
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM256
Purity Protein A purified from animal origin-free supernatant
UniProt P49023
Gene ID 5829
Applications Immunocytochemistry : 1:1000-1:2000
Western blot : 1:1000-1:2000
Immunohistochemistry (FFPE) : 1:1000
Limitations This recombinant Paxillin antibody is available for research use only.
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Description

This recombinant Paxillin antibody reacts to human Paxillin. It may also react to mouse or rat Paxillin, as predicted by immunogen homology.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Paxillin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A peptide corresponding to the N-terminus of human Paxillin was used as the immunogen for this recombinant Paxillin antibody.

Storage

Store the recombinant Paxillin antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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