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Home >> Antibodies >> Recombinant p53 Antibody / TP53

Recombinant p53 Antibody / TP53 [clone rTP53/1739] (V3939)

  Catalog No Formulation Size Price (USD)  
Image V3939-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3939-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3939SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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Western blot testing of human HeLa cell lysate with recombinant p53 antibody (clone rTP53/1739). Expetected molecular weight ~53 kDa.
IHC testing of FFPE human breast carcinoma stained with recombinant p53 antibody (clone rTP53/1739). Required HIER: boil tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant p53 antibody (clone rTP53/1739). These results demonstrate the foremost specificity of the rTP53/1739 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free recombinant p53 antibody (clone rTP53/1739) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rTP53/1739
Purity Protein G affinity chromatography
UniProt P04637
Localization Nuclear
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations This recombinant p53 antibody is available for research use only.
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Description

Recognizes a 53 kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53 under denaturing and non-denaturing conditions. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.

Application Notes

Optimal dilution of the recombinant p53 antibody should be determined by the researcher.

Immunogen

Recombinant full length human protein was used as the immunogen for the recombinant p53 antibody.

Storage

Store the recombinant p53 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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