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Home >> Antibodies >> Recombinant NKX2.2 Antibody / Rabbit Monoclonal

Recombinant NKX2.2 Antibody / Rabbit Monoclonal [clone NX2/1422R] (V7271)

  Catalog No Formulation Size Price (USD)  
V7271-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 379

V7271-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199

V7271SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 399

V7271IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 379
IHC testing of FFPE Ewings sarcoma with recombinant NKX2.2 antibody (clone NX2/1422R). HIER: steam sections in 10mM citrate buffer, pH 6.0, for 10-20 min.
SDS-PAGE analysis of purified, BSA-free recombinant NKX2.2 antibody (clone NX2/1422R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human. Other species not known.
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, k
Clone Name NX2/1422R
Purity Protein A affinity chromatography
Gene ID 4821
Localization Nuclear
Applications Flow Cytometry : 0.5-1ug/10e6 cells in 0.1ml
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This recombinant NKX2.2 antibody is available for research use only.
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Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. Antibody to NKX2.2 detects a valuable marker for Ewing sarcoma, with a sensitivity of 93% and a specificity of 89%, and aids in the differential diagnosis of small round cell tumors.

Application Notes

Titering of the recombinant NKX2.2 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.


A human partial recombinant protein was used as the immunogen for this recombinant NKX2.2 antibody.


Store the recombinant NKX2.2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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