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Home >> Antibodies >> Recombinant Multi Cytokeratin Antibody / Rabbit Monoclonal

Recombinant Multi Cytokeratin Antibody / Rabbit Monoclonal [clone KRT/1877R] (V3699)

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  Catalog No Formulation Size Price (USD)  
Image V3699-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3699-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3699SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3699IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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Immunofluorescent staining of methanol-fixed human MCF7 cells with recombinant Multi Cytokeratin antibody antibody (clone KRT/1877R, green) and Reddot nuclear stain (red).
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Immunofluorescent staining of methanol-fixed human MCF7 cells with recombinant Multi Cytokeratin antibody antibody (clone KRT/1877R, green) and Reddot nuclear stain (red).
IHC staining of FFPE human skin with recombinant Multi Cytokeratin antibody (clone KRT/1877R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant Multi Cytokeratin antibody (clone KRT/1877R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name KRT/1877R
Purity Protein A affinity chromatography
Localization Cytoplasmic
Applications Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This recombinant Multi Cytokeratin antibody is available for research use only.
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Description

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This mAb recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 53kDa (CK13) and 45kDa (CK18). This is a broad-spectrum mAb which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Application Notes

Optimal dilution of the recombinant Multi Cytokeratin antibody should be determined by the researcher.

1.The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A Keratin-enriched preparation from cultured human epithelial cells was used as the immunogen for the recombinant Multi Cytokeratin antibody.

Storage

Store the recombinant Multi Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Related Products

   1.   View all Multi Cytokeratin antibodies

   2.   View all recombinant antibodies

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