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Home >> Antibodies >> Recombinant mTOR Antibody

Recombinant mTOR Antibody [clone RM274] (R20291)

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  Catalog No Formulation Size Price (USD)  
Image R20291-0.1ML Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ul 419
Microvalidated Recrabbitmono
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Western blot of human HeLa cell lysate using recombinant mTOR antibody at 1:1500. Predicted molecular weight ~280 kDa, also observed at ~220 kDa.
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Western blot of human HeLa cell lysate using recombinant mTOR antibody at 1:1500. Predicted molecular weight ~280 kDa, also observed at ~220 kDa.
IHC testing of FFPE human breast cancer tissue with recombinant mTOR antibody at 1:1000.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse, Rat
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM274
Purity Protein A purified from animal origin-free supernatant
UniProt P42345
Gene ID 2475
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1:500-1:1000 (1)
Western blot : 1:1000-1:2000
Limitations This recombinant mTOR antibody is available for research use only.
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Description

This recombinant mTOR antibody reacts to human serine/threonine-protein kinase mTOR. It may also react to mouse or rat mTOR, as predicted by immunogen homology.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant mTOR antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

A peptide corresponding to the human serine/threonine-protein kinase mTOR was used as the immunogen for this recombinant mTOR antibody.

Storage

Store the recombinant mTOR antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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