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Home >> Antibodies >> Recombinant Mouse IgG1 Antibody

Recombinant Mouse IgG1 Antibody [clone RM106] (R20164)

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  Catalog No Formulation Size Price (USD)  
Image R20164-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 419
Microvalidated Recrabbitmono
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Western blot of nonreduced(-) and reduced(+) mouse IgG1 (20ng/lane), using 0.2ug/mL of recombinant Mouse IgG1 antibody. This mAb reacts to nonreduced IgG1 (~150 kDa) stronger than the reduced γ1 form (~50 kDa).
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Western blot of nonreduced(-) and reduced(+) mouse IgG1 (20ng/lane), using 0.2ug/mL of recombinant Mouse IgG1 antibody. This mAb reacts to nonreduced IgG1 (~150 kDa) stronger than the reduced γ1 form (~50 kDa).
ELISA of mouse immunoglobulins shows recombinant Mouse IgG1 antibody reacts to the Fc region of mIgG1; slightly cross reacts to IgG2b; no cross reactivity with IgG2a, IgG3, IgM, IgA, IgE, human/rat IgG.
ELISA titer: the plate was coated with different amount of mouse IgG1. A serial dilution of recombinant Mouse IgG1 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
Availability 1-3 business days
Species Reactivity Mouse
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM106
Purity Protein A purified from animal origin-free supernatant
UniProt P01868
Gene ID 16017
Applications ELISA : 0.005-0.2ug/ml
Western Blot : 0.1-0.5ug/ml
Limitations This recombinant Mouse IgG1 antibody is available for research use only.
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Description

This recombinant Mouse IgG1 antibody reacts to the Fc region of mouse IgG1. No cross reactivity with IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. It may slightly cross react to mIgG2b, and may also react to goat IgG.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgG1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Mouse IgG was used as the immunogen for this recombinant Mouse IgG1 antibody.

Storage

Store the recombinant Mouse IgG1 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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