- Tel: 858.663.9055
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Recombinant MITF Antibody [clone MPAF3-2R] (V7455)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V7455-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 519 | |
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V7455-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 229 | |
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V7455SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 519 | |
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V7455IHC-7ML | Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* | 7 ml | 519 |
|
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Clone Name | MPAF3-2R |
| Purity | Protein A affinity chromatography |
| UniProt | O75030 |
| Localization | Nuclear |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT Prediluted IHC only format : incubate for 30 min at RT (1) |
| Limitations | This recombinant MITF antibody is available for research use only. |
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MITF (microphthalmia transcription factor) is a transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. [UniProt]
The stated application concentrations are suggested starting points. Titration of the recombinant MITF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Recombinant full-length human protein was used as the immunogen for the recombinant MITF antibody.
Store the recombinant MITF antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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